Janine Prange-Kiel

Associate Professor

Department of Cell Biology, UT Southwestern

5323 Harry Hines Blvd, 75390-8587

214-648-3117

E-mail: [email protected]

1. Education and Training

8/88-8/94     M.S. Biology, University of Muenster, Germany, Botanical Systematic, Dr. F. Albers, advisor

7/95-3/98     Ph.D. Cell Biology, University of Tuebingen, Germany, Reproductive Cell Biology, Dr. L. Kiesel, advisor

8/01-1/06     “Privat-Dozent” Habilitation (2. Thesis) Anatomy, University of Hamburg, Germany, Effects of Estrogen

2. Positions

 1995-1997         Graduate Student, Obstetrics and Gynecology, University Tuebingen,

                              Germany

 1998-2001         Instructor and Scientist, Institute of Anatomy, University of Greifswald, Germany

 2001-2003         Instructor and Scientist, Institute of Anatomy, University of Hamburg, Germany

 2002-2003         Visiting Research Fellow, Obstetrics and Gynecology, Yale University, CT

 2003-2008         Senior Scientist and Instructor, Institute of Anatomy, University of Hamburg, Germany

 2008-2017         Assistant Professor, Cell Biology/Gross Anatomy, UT Southwestern, TX

 2017-2021         Associate Professor, Cell Biology/Gross Anatomy, UT Southwestern, TX

 2021-present     Associate Professor, Surgery/Section of Anatomy, UT Southwestern, TX

3. Honors

2002         Fellowship of the German Research Foundation

2009         Alzheimer’s Disease Research Award of The Friends of Alzheimer’s Disease Center

                  and UTSouthwestern

4. Peer-reviewed publications (in chronological order)

1. Prange-Kiel J, Rune GM, Wallwiener D, Kiesel L. (2000) Inhibition of proliferation and differentiation by RU 486 in human endometrial stromal and epithelial cells in vitro. Exp Clin Endocrinol Diabetes 108:275-281.
2. Wehrenberg U, Prange-Kiel J, Rune GM (2001) Steroidogenic factor-1 (SF-1) expression in marmoset and rat hippocampus: co-localization with StAR and aromatase. J Neurochem 76: 1879-1886.
3. Prange-Kiel J, Rune GM, Zwirner M, Wallwiener D, Kiesel L. (2001) Regulation of estrogen receptor alpha and progesterone receptor (isoform A and B) expression in cultured human endometrial cells. Exp Clin Endocrinol Diabetes 109:231-237.
4. Prange-Kiel J, Kreutzkamm C, Wehrenberg U, Rune GM. (2001) Role of tumor necrosis factor in preovulatory follicles of swine. Biol Reprod 65:928-935.
5. Rune GM, Wehrenberg U, Prange-Kiel J, Zhou L, Adelmann G, Frotscher M. (2002) Estrogen up-regulates estrogen receptor alpha and synaptophysin in slice cultures of rat hippocampus. Neuroscience 113:167-175.
6. Prange-Kiel J, Wehrenberg U, Jarry H, Rune GM. (2003) Para/autocrine regulation of estrogen receptors in hippocampal neurons. Hippocampus 13:226-234.
7. Prange-Kiel J, Rune GM, Leranth C. (2004) Median raphe mediates estrogenic effects to the hippocampus in female rats. Eur J Neurosci 19:309-317.
8. Leranth C, Prange-Kiel J, Frick KM, Horvath TL. (2004) Low CA1 spine synapse density is further reduced by castration in male non-human primates. Cereb Cortex 14:503-510.
9. Frick KM, Fernandez SM, Bennett JC, Prange-Kiel J, MacLusky NJ, Leranth C. (2004) Behavioral training interferes with the ability of gonadal hormones to increase CA1 spine synapse density in ovariectomized female rats. Eur J Neurosci 19:3026-3032.
10. Kretz O, Fester L, Wehrenberg U, Zhou L, Brauckmann S, Zhao S, Prange-Kiel J, Naumann T, Jarry H, Frotscher M, Rune GM. (2004) Hippocampal synapses depend on hippocampal estrogen synthesis. J Neurosci 4:5913-5921.
11. Parducz A, Hajszan T, MacLusky NJ, Hoyk Z, Csakvari E, Kurunczi A, Prange-Kiel J, Leranth C. (2006) Synaptic remodelling induced by gonadal hormones. Neuronal plasticity as a mediator of neuroendocrine and behavioural responses to steroids. Neurscience, 138:977-985.
12. Prange-Kiel J, Rune GM. (2006) Direct and indirect effects of estrogen in the hippocampus.  Neuroscience, 138:765-772.
13. MacLusky NJ, Hajszan T, Prange-Kiel J, Leranth C. (2006) Androgen modulation of hippocampal synaptic plasticity. Neuroscience, 138:957-965.
14. Prange-Kiel J, Fester L, Zhou L, Lauke H, Carretero J, Rune GM. (2006) Inhibition of hippocampal estrogen synthesis causes region-specific downregulation of synaptic protein expression in hippocampal neurons. Hippocampus 24:464-471.
15. Fester L, Ribeiro-Gouveia V, Prange-Kiel J, von Schassen C, Böttner M, Jarry H, Rune GM. (2006) Proliferation and apoptosis of hippocampal granule cells require local oestrogen synthesis. J Neurochem, 97:1136-1144.
16. Rune GM, Lohse C, Prange-Kiel J, Fester L, Frotscher M. (2006) Synaptic plasticity in the hippocampus: Effects of estrogen from the gonads or hippocampus. Neurochem Res, 31:145-155.
17. von Schassen C, Fester L, Prange-Kiel J, Lohse C, Huber C, Böttner M, Rune GM. (2006) Oestrogen synthesis in the hippocampus: Role in axon outgrowth. J Neuroendocrinol 18:847-856.
18. Prange-Kiel J, Jarry H, Schoen M, Kohlmann P, Lohse C, Zhou L, Rune GM. (2008) Gonadotropin-releasing hormone regulates spine density via its regulatory role on hippocampal estrogen synthesis. JCB 180:417-426
19. Fester L, Zhou L, Butow A, Huber C, von Lossow R, Prange-Kiel J, Jarry H, Rune GM. (2009) Cholesterol-promoted synaptogenesis requires the conversion of cholesterol to estradiol in the hippocampus. Hippocampus, 19:692-705.
20. Prange-Kiel J, Fester L, Zhou L, Jarry H, Rune GM (2009) Estrus cyclicity of spinogenesis: underlying mechanisms. J Neural Transm., 116:1417-1425.
21. Fester L, Zhou L, Voets C, Ossig C, Disteldorf E, Peters C, Bläute F, Prange-Kiel J, Dudzinski D, Jarry H, Rune GM. (2009) The opposing roles of estradiol on synaptic protein expression in hippocampal cultures. Psychoneuroendocrinology, Suppl 1:S123-9.
22. Zhou L, Fester L, v. Bitersdorf B, Hassu B, Nogens H, Prange-Kiel J, Jarry H, Wegscheider K, Rune GM. (2010) Aromatase inhibitors induce spine synapse loss in the hippocampus of ovariectomized mice. Endocrinology, 151:1153-1160.
23. Renthal NE, Chien-Cheng C, Williams KC, Gerard RD, Prange-Kiel J, Mendelson CR. (2010) The miR-200 Family and Targets, ZEB1 and ZEB2, Modulate Uterine Quiescence and Contractility during Pregnancy and Labor. PNAS 107: 20828-20833.
24. Fester L, Prange-Kiel J, Jarry H, Rune GM. (2011) Estrogen synthesis in the hippocampus. Cell and Tissue Research., 345:285-94.
25. Ramakers GJ, Wolfer D, Rosenberger G, Kuchenbecker K, Kreienkamp HJ, Prange-Kiel J, et al. (2012) Dysregulation of Rho GTPases in the {alpha}pix/arhgef6 mouse model of X-linked intellectual disability is paralleled by impaired structural and synaptic plasticity and cognitive deficits. Hum Mol Genet., 21:268-86.
26. Fester L, Prange-Kiel J, Zhou L, Blittersdorf BV, Böhm J, Jarry H, Schumacher M, Rune GM. (2012) Estrogen-regulated synaptogenesis in the hippocampus: Sexual dimorphism in vivo but not in vitro. J Steroid Biochem Mol Biol., 131:24-29.
27. Prange-Kiel J, Schmutterer R, Fester L, Zhou L, Imholz P, Brandt N, Vierk R, Jarry H, Rune GM. (2013) Endocrine regulation of estrogen synthesis in the hippocampus? Prog Histochem Cytochem., 48:49-64.
28. Prange-Kiel J, Dudzinski DA, Pröls F, Glatzel M, Matschke J, Rune GM. (2016) Aromatase Expression in the Hippocampus of AD Patients and 5xFAD Mice. Neural Plasticity, Volume 2016, Article ID 9802086
29. Prange-Kiel J, Champine JG, Winkler AJ, Twickler DM. (2016) Embryology, Anatomy, and Radiology of Cervical Cysts and Cleft Lip/Palate – A TBL Module for Medical Students, MedEdPORTAL Publication. 2016;12:101484. https://doi.org/10.15766/mep_2374-8265.10484
30. Evers BM, Rodriguez-Navas C, Tesla RJ, Prange-Kiel J, Wasser CR, Yoo KS, McDonald J, Cenik B, Ravencroft TA, Plattner F, Ragemakers R, Yu G, White CL 3^rd, Herz J, (2017) Lipidomic and Transcriptomis Basis of Lysosomal Dysfunction in Progranulin Deficiency. Cell Rep., 12:2565-74.

5. Book Chapters

1. Prange-Kiel J Embryology of the Head and Neck, in: Atlas of Oral and Maxillofacial Surgery. Kademani D.; Tiwana P (Eds.) Elsevier, 2015.
2. Prange-Kiel J, Rune GM. Zur zerebralen Wirkung von Östrogenen (Cerebral effects of estrogens), in: Intersexualität kontrovers, Schweizer K; Richter-Appelt H (Eds.), Psychosozial-Verlag, 2012.
3. Prange-Kiel J, Rune GM. Auto- und parakrine Regulation im ZNS: Neurosteroide (Autocrine and paracrine regulation in the CNS, Neurosteroids), in: Molekularmedizinische Grundlagen von Endokrinopathien 2- Para- und autokrine Regulation, Ganten D; Ruckpaul K (Eds.), Thieme, 2006.
4. Albers F, Meve U, Kiel J. Illustrated Handbook of Succulent Plants: Asclepiadaceae. Springer,

     2002.

6. Other Publications

1. Prange-Kiel J. (2011) Östrogene im Gehirn (Estrogens in the brain). Gynäkologische Endokrinologie, 9:14-21.
2. Fester L, Prange-Kiel J, Rune GM. (2007) Hippokampale Estrogensynthese und synaptische Plastizität (Hippocampal estrogen synthesis and synaptic plasticity), Neuroforum 4.07:127-134.
3. Prange-Kiel J. (2006) Östrogenwirkungen im Gehirn (Action of estrogens in the brain). Gynäkologische Endokrinologie, 4:6-11.

7. Invited Seminars and Talks

1. Die Rolle von Östradiol und Aromatase in Morbus Alzheimer, June 28, 2012, University of Frankfurt, Germany
2. GnRH and estrogen synthesis in the hippocampus, Institute of Neuroanatomy, University of Hamburg, April 2^nd, 2012
3. Estrogen synthesis in the hippocampus: Is there a connection to Alzheimer's disease? Stem Cells in Neuroscience Seminar Series, UT Southwestern, Dallas, USA, December 16^th, 2011
4. Oestrogen and Alzheimer’s Disease. Friends of Alzheimer’s Disease Center at UT Southwestern, Dallas, USA, April 1^st, 2010
5. Hippocampal aromatase expression in Alzheimer's disease. 105th Annual Meeting of the Anatomische Gesellschaft, Hamburg, Germany, March 26 – 29, 2010
6. Hippocampal Estrogen: Its Function and Regulation. Stem Cells in Neuroscience Seminar Series, UT Southwestern, Dallas, USA, February 18^th, 2010
7. Estrogen Synthesis in the Hippocampus and its Role in Alzheimer's Disease. Alzheimer’s Disease Center, UT Southwestern, Dallas, USA, December 16^th, 2009
8. Function and regulation of hippocampal estrogen synthesis. New Faculty Research Forum, UT Southwestern, Dallas, USA, September 9^th, 2009
9. GnRH regulates spine density via its regulatory role in hippocampal estrogen synthesis. Spring Hippocampal Research Conference, Verona, Italy, June 14 – 19, 2009
10. Gonads and Cognition: Estrogen Regulates Synaptic Plasticity. MSTP Program, UT Southwestern, Dallas, USA, October 16^th, 2008
11. Gonads and Cognition: Estrogen Regulates Synaptic Plasticity. Midwestern University at Glendale, Arizona, USA, December 7^th, 2007
12. Synaptic Plasticity is regulated by estrogen. Department of Cell Biology, UT Southwestern, Dallas, USA, October 18^th, 2007
13. Was hat GnRH mit dem Hippocampus zu tun? Institut für Anatomie, Universität Rostock, Germany, December 16^th, 2006
14. Klinik für Frauenheilkunde und Geburtshilfe, Universitätsklinikum Aachen, Germany, January 22^nd , 2005

8. Presented Talks and Posters (last 3 years, abstracts)

1. Winkler AJ, Botterman BR, Prange-Kiel J, Champine JG, Moore DS. Osterology and Radiology of the Back and Extremities: An Integrated Exercise for a large Medical School Class. The 2019 Innovations in Health Science Education Annual Conference (Shine Academy), Austin, TX February 21 – 22, 2019
2. Bishop K, Prange-Kiel J, Winkler A, Champine J. Incorporating Radiology into the Undergraduate Medical School Preclinical Team Based Learning Curriculum. The 2019 Innovations in Health Science Education Annual Conference (Shine Academy), Austin, TX February 21 – 22, 2019
3. Bishop K, Prange-Kiel J, Winkler A, Champine J. Incorporating Radiology into the Undergraduate Medical School Preclinical Team Based Learning Curriculum. 66^th Annual Meeting of the Association of University Radiologists, Orlando, FL, May 7 – 10, 2018
4. Winkler AJ, Botterman BR, Prange-Kiel J, Champine JG, Moore DS. Osterology and Radiology of the Back and Upper Extremity: An Integrated Exercise for a large Medical School Class. Annual Meeting of the American Association of Anatomists at Experimental Biology, San Diego, CA, April 21 – 25, 2018
5. Prange-Kiel J, Champine JG, Winkler AJ, Twickler DM. Developing a Team-Based Learing Module for large classes in Medical School. Annual Meeting of the American Association of Anatomists at Experimental Biology, Chicago, IL, April 22 – 25, 2017

1. Research Support

Current Project

Southwestern Academy of Teachers (SWAT)

Effects of “The Origami Embryo”, a hands-on exercise, on the understanding of early embryonic development.

07/01/2018-06/28/2019, Small Grant Award, $1,411

Previous Projects

Friends of Alzheimer’s Disease Center at University of Texas Southwestern Medical Center at

Dallas

Hippocampal Aromatase Expression in Alzheimer’s Disease

04/01/2009-03/31/2010, Pilot Project Grant, $ 65,000

Role: PI

German Research Foundation PR 703/2-1

Regulation of hippocampal estradiol synthesis via GnRH

03/01/2008-02/28/2010, Research Grant, approx. $ 150,000

Role: PI

In cooperation with Hubertus Jarry, University of Goettingen, Germany

This national Research Grant was not transferable to the US, research continues in Germany

German Research Foundation PR 703/1-1

Does estradiol regulate the activity of hippocampal pyramidal cells via projecting neurons of the median raphe?

11/01/2002-10/31-2003, Research Fellowship, approx. $ 60,000

Role: PI

10. Department and University Committees and Service

2005-2008        Examiner for the 1. Medical State Examine (“Physikum”), Germany, approx. 50

                            exams per year

2005-2008        Board Member of the “Schwerpunkt Neurowissenschaften”, a society to support

                            and coordinate neuroscience at the University of Hamburg, Germany

2006-2008        Member of several Dissertation Committees, University of Hamburg, Germany

2006-2008        Member of several Search Committees, University of Hamburg, Germany

2008-present    Faculty Member of the Neuroscience Graduate Program (participating in seminars

                                     and qualification exams)

2008-present    Admission Interviewer for the Medical School at UT Southwestern, TX (12

                            interviews per year)

2008-2014        Admission Interviewer for the Graduate School at UT Southwestern, TX (4

                            interviews per year)

2009-present    Faculty Member of the STARS Program, UT Southwestern, TX

10/2009                      Judge for UT Southwestern Sigma Xi graduate student abstract competition

02/2010             Judge for 7th Annual Postdoctoral Poster Session & Symposium

 10/2011              Judge for the UT Southwestern Graduate Student Organisation Poster Session

2012-2015        Member of the MS1 Course Director Committee

2013-present    Admission Interviewer for the UT-PACT Program (4 interviews per year)

 2013-present     Member of the Student Promotion Committee

 2014-present     Member of the Six-Year Plan - Medical Education Subcommittee

 2014-present     Admission Interviewer for JAMP (4 interviews per year)

 2014-2015         Member of various focus groups for UT Southwestern Medical School Curriculum    

                              Reform

 2015-present     Member of the Pre-Clerkship Course Director Committee

 2016-present     Member of the TBL Focus Group

 2017-2018         Member of the LCME Faculty Committee (Institutional Self-Study Report for

     upcoming side-visit)

11. Outside reviewing

2001-present    Peer-reviewer for Scientific Journals

BBA Molecular Basis of Disease

BMC Neuroscience

Brain Research

Cells Tissues Organs

Endocrine Research

Experimental Brain Research

Gynecological Endocrinology

Journal of Neurobiology/Developmental Neurobiology

Journal of Neuroendocrinology

Journal of Steroid Biochemistry and Molecular Biology

Neurobiology of Aging

Neuroscience

Neuroscience Letters

PLOS ONE

                           Psychoneuroendocrinology

                           Synapse

                           Trends in Endocrinology & Metabolism

2007-present    Peer-reviewer for Grant Organizations

                            FORUN, University Rostock, Germany

                            National Science Foundation, USA

50. Professional Society Membership

2000-present    Anatomische Gesellschaft

2002-present    German Neuroscience Society

2004-present    Society for Neuroscience

2009-2013        Endocrine Society

2016-present    American Association of Anatomists

1000. Professional Development

 1-12/2019          Participant in the Leadership Emerging in Academic Departments (LEAD) Program

14. Teaching Experience

1998-2001        Instructor, University of Greifswald, Medical School:

                                               Gross anatomy, laboratory (one complete course/year)

                                               Gross anatomy, seminar (one complete course/year)

                                               Histology, laboratory (one complete course/year)

2001-2008        Instructor, University of Hamburg, Medical School

                                             Lectures in gross anatomy, histology, neuroanatomy, and embryology       

                                             Gross anatomy, laboratory (one complete course/year)

                                             Gross anatomy, clinical seminar (one complete course/year)

                                             Neuroanatomy, combined laboratory and seminar (one complete

                                             course/year), histology, laboratory (one complete course/year)

2008-present    Faculty, UT Southwestern, Medical School

2012-2015        Course Director, Embryology, UT Southwestern, Medical School

2015-present    Co-Course Director, Human Structure, UT Southwestern, Medical School

Recent teaching activities:

• Didactic lectures on various topics (e.g., gross anatomy of the head, thorax, abdomen; early embryology, organogenesis, integrated lectures on anatomy, embryology, and radiology)
• Dissection laboratory (full body dissection)
• Development of embryology online curriculum on two different learning platforms
• Development of course syllabi
• Development of video lectures
• Development and conduction of exams
• Development of Team-Based Learning modules
• Development of a hands-on exercise on gastrulation and neurulation

O. Teaching Awards

2012                         UT Southwestern Pre-Clinical Teaching Award (SWAT): Anatomy

2013                         UT Southwestern Pre-Clinical Teaching Award (SWAT): Embryology

2015             Outstanding Teacher Award (UT Southwestern Medical School, Class of 2019)

2016             Favorite Course in MS 2020 (Human Structure, UT Southwestern Medical School,

                      Class of 2020)

P. Supervisory and Training Experience

2006-present     Graduate Medical Students (M.D. Theses)